Fig. 3. The effects of high expression ZNF132 on characteristics of esophageal cancer cell lines in vitro.

a, c Expression of ZNF132 measured by q-PCR and western blotting in Ec-109 and CaEs-17 cells. b, d Effect of ZNF132 gene on the proliferation of esophageal cancer cells by cell proliferation assay. Light absorbance measurement at a wavelength of 450 nm was recorded to show that upregulation of ZNF132 gene could inhibit the proliferation of CaEs-17 and Ec-109 cells. e, f In vitro scratch healing experiment showed that high expression of ZNF132 in Ec-109 and CaEs-17 cells significantly inhibits the ability of the cells to heal. g The upregulation of ZNF132 gene in Ec-109 and CaEs-17 cells reduced cell migration ability in a transwell assay. h Flow cytometry demonstrates that upregulation of ZNF132 in CaEs-17 and Ec-109 cells could significantly increase the cell apoptosis rate. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001