Fig. 4. Overexpression of ZNF132 inhibits the growth of human esophageal squamous cell carcinoma in vivo in a mouse xenograft model.

a Small tumor volume with pCD513B-ZNF132 transection. Ec-109 cells transfected with either the plasmid pCD513B-ZNF132 or pCD513B were inoculated into the BALB/c nude mice. Tumor volume and mouse body weight were measured throughout the procedure. When the tumor reached the expected standard, the mice were sacrificed and the tumor was dissected, recorded, and the body wet weight of the tumor was measured. b, c It was evident from tumor volume (b) and tumor wet weight (c) that the overexpression of ZNF132 gene could significantly inhibit the tumorigenic ability of Ec-109 cells. *P < 0.05, ***P < 0.001. d Body weight in the experimental group and the control group during the whole experiment. e Western blotting results to show that ZNF132 was expressed in a subcutaneous injection of ZNF132 stable cell line