Fig. 4.
iNKT and γδ-T cells are a major source of T cell derived IL-17 in SpA joints. a FlowSOM visualization of iNKT and γδ-hi T cells present in synovial fluid samples from three treatment naïve SpA patients, stained for the indicated markers set (as explained in Fig. 3F). Nodes show cell subsets (including RORγt + cells) enriched in the synovial fluid (compared to cells from paired blood samples). Other subsets (nodes) are present but at a relatively lower frequencies. b Paired analyses of IL-17 production by SpA SF and blood derived iNKT and γδ-T cells as determined in Fig. 3G. (*p < 0.05, paired t-tests). c Synovial fluid derived mononuclear cells (SFMC) and SFMC cells depleted of iNKT and TCRγδ cells (ΔSFMC, illustrated in the plots) were cultured in the presence or absence of IL-23 combined with or without aCD3Ab/aCD28Ab stimulation. Supernatants were collected at 72 h of culture and cytokines production was measured by ELISA (SFMC vs. ΔSFMC; *p < 0.05, **p < 0.01 two-way ANOVA). Results from one experiment are shown (Representative for a total of three independent experiments using SF samples from 3 SpA patients). SFMC data (white bars), ΔSFMC (black bars). Data throughout this figure are presented as mean ± SEM