Fig. 8. CUL1 inducing cytokines activated PI3K–AKT signaling pathway.

a The expressions of PI3K–AKT–mTOR signaling pathway-related proteins, such as PI3K, p-AKT, AKT, p-mTOR, mTOR, p-P^70S6K, and P^70S6K in MDA-MB-231 cells with CUL1 knockdown or vector control. b MDA-MB-231 cells were transiently transfected with si-CUL1 and si-Ctrl for 48 h and these cells were continued culturing in serum-free medium with or without supplementary recombinant CXCL8 and IL11 proteins for 24 h, then western blotting was used to test the expressions of p-AKT, AKT, p-mTOR, mTOR, p-P^70S6K, and P^70S6K