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. 2018 Dec 18;10(1):4. doi: 10.1038/s41419-018-1207-4

Fig. 3. PTENP1 and PTEN are target genes of miR-200c.

Fig. 3

a, b http://www.microrna.org and ClustalW2/RNA structure was predicted that PTEN or PTENP1 have bound to miR-200c directly. c miR-200c mimics were co-transfected with the PTEN wt 3′UTR or mt 3′UTR. Luciferase activities were used to detect the effect on PTEN expression at 48 h after transfection. d miR-200c mimics were co-transfected with the PTENP1 wt 3′UTR, mt 3′UTR-1, mt 3′UTR-2, or mt 3′UTR-1+2 as indicated. Luciferase activities were used to detect the effect on PTENP1 expression at 48 h after transfection. e Co-transfection dual-luciferase reporter by transfecting RL-952 cells with miR-200c mimics to verify the interaction between the PTENP1, PTEN, and miR-200c. f MiR-200c and PTENP1 palsmid co-transfection with RL-952 cells to detected the change of PTEN protein in western blot. g The interaction with microRNA-200c and PTEN was assayed using the method of AGO2-RIP. PTEN can combined with Ago2 protein as well as miR-200c to form an RNA-induced silencing complex. PTEN-Ago2 were significantly higher than negative control group (IgG). All results are presented as the mean of triplicate assays. **P < 0.01