FIG 1.

Prime and boosts with PBK001 vaccine provided 100% protection with low bacterial burden in organs. (A) C57BL/6 mice were i.n. immunized with either PBS (n = 10) or strain PBK001 at 1.5 × 10⁴ CFU (n = 10). Three weeks after the last boost, the mice were challenged with B. pseudomallei K96243 strain via the aerosol route. Survival was analyzed using a log rank (Kaplan Meier) test (****, P < 0.0001). (B) Bacterial burden in lungs, livers, and spleens (n = 7) of surviving mice at day 27 postchallenge. n.s., not significant.