TABLE 1.
Bacterial strains and plasmid used in this study
| Bacterial strain | Relevant featuresa | Reference or source |
|---|---|---|
| B. pseudomallei K96243 | Human clinical isolate from Thailand; Ipms Cazs Chls Cips, Augmentins Minr Genr Tmp-Smxr Strr | 56 |
| B. pseudomallei PBK001 | B. pseudomallei CLH010 with an unmarked intragenic deletion in BPSS0368 (ΔtonB) | This study |
| B. pseudomallei CLH010 | B. pseudomallei K96243 with an unmarked intragenic deletion in BPSS1498 (Δhcp1) | This study |
|
E. coli S17-1 λpir (pMo130-ΔBPSS1498) |
Donor strain containing pMo130-Δbpss1498 plasmid; Smr Tpr Pbs Kmr | 23 |
a
Ipm, imipenem; Caz, ceftazidime; Chl, chloramphenicol; Cip, ciprofloxacin; Augmentin, amoxicillin/clavulanate potassium; Min, minocycline; Gen, gentamicin; Tmp-Smx, trimethoprim-sulfamethoxazole; Str, streptomycin; Km, kanamycin; Pb, polymyxin B; Sm, streptomycin; Tp, trimethoprim.