Figure 3.

EGFR-Mutant Lung Tumors Lacking the RAS-PI3K Interaction Show Strongly Delayed Development

(A) Volume of air remaining in mouse lungs after tamoxifen (2 weeks) and doxycycline diet at different time points (n = 26 for Pik3ca^WT/− and n = 21 for Pik3ca^MUT/−).

(B) Representative examples of lungs visualized by micro-CT scan (left), ex-vivo view (middle), and histological sections stained with H&E after 18 weeks of a doxycycline diet.

(C) Quantification of the number of tumor foci per lung after 18 weeks of a doxycycline diet from histological samples (n = 7 per group).

(D) Quantification of the area of the lung occupied by tumor foci after 18 weeks of a doxycycline diet from histological samples (n = 7 per group).

(E) Representative examples of proliferation in tumor samples visualized by Ki-67 staining. Scale bar, 100 μm.

(F) Quantification of proliferation by Ki-67 staining (n = 6 per group).

(G) Survival assay. The endpoint was dictated by defined welfare severity limits: moderately increased respiratory rate and/or moderately hunched appearance (n = 11 for Pik3ca^WT/Flox, n = 11 for Pik3ca^WT/−, and n = 15 for Pik3ca^MUT/−).