Skip to main content
. 2018 Dec 18;25(12):3504–3518.e6. doi: 10.1016/j.celrep.2018.11.069

Figure 1.

Figure 1

Single-Cell Expression Profiling of Prostate Cells from HN and Castrated Mice

(A) Schematic diagram demonstrating experimental setup for single-cell expression profiling.

(B) FACS gating strategy used to sort single prostate cells, from Lineage-negative cells (Lin−: CD31−CD45−TER119−).

(C) Quantification of keratin expression profiles by IF based on three FACS-sorted populations as in (B). The number of cells counted in each group is shown from five mice. See also Figures S1A–S1C.

(D) Hierarchical clustering of single prostate cells from HN and castrated (CR) mice showing separation of luminal, basal, and stromal cells, as well as the luminal (white), basal (yellow), bi-lineage (red), and stromal (purple) gene sets. Color scale is indicated. See also Table S2.

(E) Enlarged view of select genes from the heatmap in Figure S1I clustering prostate cells in the luminal lineage from HN mice into five subsets. Color scale is indicated.

See also Figure S1.