Figure 3.

Characterization of LY6D⁺ Prostate Epithelial Subpopulations from HN Mice

(A) FACS analysis of Lin⁻ cells from prostates (from HN mice) confirmed LY6D expression in basal (SCA1^int), intermediate (SCA1^high), and a small portion of luminal (SCA1^low/−) cells. n = 3 for each sorted subpopulation. p values: ^∗∗p < 0.01 and ^∗∗∗p < 0.001. Error bars represent mean ± SEM.

(B) Quantification of keratin expression by IF staining of the FACS-sorted subpopulations as in (A). The number of cells counted in each group is shown from four mice. See also Figure S3A.

(C) FACS analysis of LY6D⁺ cells from HN prostates for their staining patterns of select prostate stem/progenitor and luminal surface markers.

(D) Quantification of marker profiles based on FACS subpopulations as in (C). The average of frequency of the indicated populations is from three mice.

(E) Comparison of the gene expression profiles from LY6D⁺ and LY6D⁻ subpopulations (based on RNA-seq data) by principal-component analysis (PCA).

(F) Heatmap of differentially expressed genes in sorted LY6D⁺ and LY6D⁻ subsets from the SCA1^{high or int or low/−} populations. Log2 expression values (based on microarray) were normalized to luminal (SCA1^low/−) LY6D⁻ subset for each gene (=0).

See also Figure S3.