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. 2018 Dec 26;25(13):3661–3673.e3. doi: 10.1016/j.celrep.2018.11.098

Figure 2.

Figure 2

Ectopic Expression of HuntingtinQ128, but Not α-SynucleinA30P, Induces an Upregulation of the FlowerLoseB Isoform

(A and B) Expression of the flowerubi::YFP, flowerLoseA::GFP, flowerLoseB::RFP reporter in the optic lobe of adult flies (A), and quantification (B) of the RFP-positive cells (red) for the following genotypes: GMR>lacZ, GMR>httQ0, and GMR>httQ128. DAPI is shown in blue, and GFP is shown in green. Scale bar: 10 μm.

(C and D) Quantification (D) of the number of apoptotic cells in the eye discs of third-instar larva expressing UAS-lacZ, UAS-httQ0, or UAS-httQ128 under the control of GMR-Gal4 driver, and representative figures for each genotype (C). Apoptotic cells are marked by DCP1 in green, and nuclei are shown in blue. Scale bar: 10 μm.

(E and F) Expression of the flowerubi::YFP, flowerLoseA::GFP, flowerLoseB::RFP reporter in the optic lobe of adult flies (E), and quantification (F) of RFP-positive cells (red) for the following genotypes: GMR>lacZ, GMR > α-synWT, and GMR > α-synA30P. DAPI is shown in blue, and GFP is shown in green. Scale bar: 10 μm.

(G and H) Quantification (H) of the number of apoptotic cells in the eye discs of third-instar larva expressing UAS-lacZ, UAS-α-synWT, or UAS-α-synA30P under the control of GMR-Gal4, and representative figures (G) for each genotype. Apoptotic cells are marked by DCP1 in green, and nuclei are represented in blue. Scale bar: 10 μm.

(I and J) Expression of the flowerubi::YFP, flowerLoseA::GFP, flowerLoseB::RFP reporter in the optic lobe of adult flies (I), and quantification (J) of RFP-positive cells (red) for the following genotypes: GMR>lacZ, GMR > Aβ40, and GMR > Aβ42. DAPI is shown in blue, and GFP is shown in green. Scale bar: 10 μm.

(K and L) Quantification (L) of the number of apoptotic cells in the eye discs of third-instar larva expressing UAS-lacZ, UAS-Aβ42, or UAS-Aβ42/UAS-diap1 under the control of GMR-Gal4, and representative figures for each genotype (K). Apoptotic cells are marked by DCP1 in green, and nuclei are represented in blue. Scale bar: 10 μm.

Error bars indicate SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. The number of positive cells for the genotype GMR>lacZ was assumed to be 100% for normalization. See also Figure S2.