Antibodies |
|
rat anti-ELAV |
DSHB |
Cat#7E8A10; RRID: AB_528218
|
polyclonal anti-Myc-tag |
Cell Signaling |
Cat#2272; RRID: AB_10692100
|
monoclonal mouse anti-Aβ42 |
Synaptic Systems |
Cat#218 721; RRID: AB_2619923
|
anti-Fasciclin II |
DSHB |
Cat#1D4; RRID: AB_528235
|
chicken anti-GFP |
Abcam |
Cat#ab13970; RRID: AB_300798
|
phalloidin Alexa-546 |
Molecular Probes |
Cat#A22283; RRID: AB_2632953
|
cleaved DCP1 |
Cell Signaling |
Cat#9578; RRID: AB_2721060
|
|
Chemicals and Commercial Assays |
|
Terminal Transferase, recombinant (rTdT) |
Roche |
Cat#3333574001 |
Biotin-16-dUTP |
Roche |
Cat#11093070910 |
RU486 (Mifepristone) |
Sigma |
Cat#M8046-1G |
propidium iodide |
Sigma |
Cat#P4170 |
proteostat aggresome dye |
Enzo Life Sciences |
Cat#ENZ-51035-0025 |
|
Experimental Models: Fly stocks |
|
UAS-Aβ42 (2x) |
Casas-Tinto et al., 2011 |
N/A |
GMR-Gal4 |
Bloomington |
BDSC:1104 |
elav-GeneSwitch |
Bloomington |
BDSC: 43642 |
azot{KO; GFP} |
Merino et al., 2015 |
N/A |
azot{KO; w+} |
Merino et al., 2015 |
N/A |
azot{KO; hid} |
Merino et al., 2015 |
N/A |
UAS-diap1 |
Bloomington |
BDSC: 6657 |
UAS-flowerLoseB
|
Rhiner et al., 2010 |
N/A |
flowerLoseA/BRNAi (long hairpin)
|
Merino et al., 2013 |
N/A |
UAS-flower RNAi kk |
VDRC |
VDRC: 104993 |
UAS- azot RNAi GD
|
VDRC |
VDRC: 18166 |
UAS- azot RNAi kk
|
VDRC |
VDRC: 102353 |
UAS-secEMAP |
Casas-Tintó et al., 2015 |
N/A |
UAS-Aβ40 |
Bloomington |
BDSC: 64215 |
UAS-httQ0 and UAS-httQ128
|
Lee et al., 2004 |
N/A |
UAS-αsynWT and UAS-αsynA30P
|
Bloomington |
BDSC: 8146 BDSC: 8147 |
flowerubi-YFP,flowerLoseA-GFP,flowerLoseB-RFP and flowerubi-flag, flowerLoseA-HA, flowerLoseB-myc |
Yao et al., 2009 |
N/A |
UAS-lacZ |
Bloomington |
BDSC: 3955 |
act > y+ > gal4 |
Rhiner et al., 2010 |
N/A |