Figure 3.

TC83 induces cell cycle delay in U87MG cells which can be relieved by a capsid-importin-α inhibitor. (A) U87MG cells were synchronized via serum-starvation (0.5% FBS) for 72 h. Cells were then infected (MOI 10) with wild-type TC83, TC83_Cm, or mock-infected for 1 h then released in complete media containing 10% FBS. Cells were collected at 16 and 24 hpi, fixed and stained with PI then analyzed for cell cycle by flow cytometry. The average of three biological replicates is displayed. ^*Statistical significance compared to mock-infected samples, ^$Significance compared to TC83 infected cells. ^*p < 0.05, ^***p < 0.001, ^$p < 0.05 (B) Similar to (A), U87MG cells were serum starved (0.1% FBS), treated with DMSO or G281-1485 (10 μM) for 1 h, infected (MOI 10) with wild-type TC83 or mock infected, and post-treated with DMSO or G281-145 in complete media containing 10% FBS. Cells were collected 24 h post-infection and analyzed by flow cytometry. The average of three biological replicates is displayed. ^*Statistical significance compared to mock-DMSO samples, ^$Significance compared to TC83-DMSO cells. ^**p < 0.01, ^***p < 0.001. ^$p < 0.05, ^$$p < 0.01.