Figure 6.

Cyclin protein expression and Rb phosphorylation are decreased following VEEV infection. (A) U87MG cells were synchronized via serum-starvation for 72 h. Cells were then infected (MOI 10) with VEEV TC83 or mock-infected for 1 h and then released in complete media containing 10% FBS. Cells were collected at 16 and 24 hpi for western blot analysis of cyclin D1, E2, A1 or actin expression. (B) Western blot analysis for phospho-Rb, capsid and actin levels in VEEV TC83 or mock infected U87MG cells.