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Comparison between directly and HaloTag coupled microspheres after incubation with commercially available animal anti-non-HLA antibodies. For AT1R and PECR only microspheres with direct coupling were available, therefore the comparison with HaloTag coupled microspheres cannot be depicted. The MFI measured after incubation of the AT1R microspheres with the anti-AT1R antibody was 13,760 and 18,842 for the PECR microspheres after incubation with the anti-PECR antibody.
