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. 2018 Dec 19;9:3002. doi: 10.3389/fimmu.2018.03002

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Copyright © 2018 Kamburova, Kardol-Hoefnagel, Wisse, Joosten, Allebes, van der Meer, Hilbrands, Baas, Spierings, Hack, van Reekum, van Zuilen, Verhaar, Bots, Drop, Plaisier, Meeldijk, Bovenschen, Seelen, Sanders, Hepkema, Lambeck, Bungener, Roozendaal, Tilanus, Voorter, Wieten, van Duijnhoven, Gelens, Christiaans, van Ittersum, Nurmohamed, Lardy, Swelsen, van der Pant, van der Weerd, ten Berge, Bemelman, van der Boog, de Fijter, Betjes, Heidt, Roelen, Claas and Otten.

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Schematic illustration of the direct and HaloTag coupling to the MagPlex microspheres. (A) We refer to direct coupling when the amino group of the protein is directly coupled to the carboxylated microsphere. (B) For the HaloTag coupling a HaloTag amine ligand serves as a connection between the carboxyl group and the HaloTag of the protein. Due to this indirect coupling, the protein of interest is freely accessible compared to the direct situation. Part of the figure is adapted from Promega.