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Schematic illustration of a part of the universal vector used for the production of HaloTag proteins. In the general pcDNA3-WPRE vector starting with a CMV promoter and including the HaloTag sequence and the WPRE sequence, which enhances expression, we ligated an insert consisting of a BglII restriction site, then a signal peptide, the DNA sequence of the protein of interest, followed by 6-Histidines and ending with an XhoI restriction site.
