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. 2018 Dec 19;9:3002. doi: 10.3389/fimmu.2018.03002

Figure 5.

Figure 5

Detection of anti-PLA2R antibodies in patient sera. (A) 18 patient sera [6 negative and 12 positive according to the qualitative anti-PLA2R indirect immunofluorescence test (IIFT)] were incubated with a microsphere mix consisting of empty microspheres and microspheres coupled with HaloTag amine ligand, Transferrin (direct-coupling), Transferrin_HaloTag (together four negative controls), coupled with IgG (positive control), and also two specific microspheres coupled with PLA2R (direct) or PLA2R_HaloTag. The MFI values of each independent patient serum are depicted including the mean and standard deviation. (B) The 12 IIFT positive patient sera can be divided into weak positive (n = 5) and positive (n = 7) according to the immunofluorescence staining. Using this stratification, we plotted the MFI values measured in our assay with the two different PLA2R microspheres (direct- and HaloTag-coupling).