FIGURE 1.

(A) 16S rRNA gene sequence phylogenetic reconstruction of strain ALE^T (bold type) and of related species in the Sporomusaceae family of the Firmicutes phylum. Consensus tree was performed after applying the maximum likelihood, neighbor-joining and maximum parsimony algorithms implemented in the ARB package. Trees were constructed with different filters (termini, subact, none) and based on 1000 replications. Bootstrap values greater than 70% are indicated by filled circles, and greater than 50% by gray circles. Bar indicates a 10% estimated sequence divergence. The sequence of Desulfosporosinus acidiphilus was used as outgroup. (B) Phylogenetic reconstruction with maximum-likehood of the core genes from 28 sequenced species closely related to Lucifera butyrica strain ALE^T. Bootstrap of 100% are indicated by filled circles, and others by their number for clarity. Bar indicates a 10% estimated sequence divergence. The sequence of Desulfosporosinus acidiphilus was used as outgroup.