Table 1.
Bacterial strains and plasmids used in this study.
| Designation | Genotypes or related characteristics | Source or reference |
|---|---|---|
| Plasmids | ||
| pUC:35S | 35S-MCS-NOS cassette in pUC19, ampicillin resistance | This study |
| pOsMAP1 | OsMAP1 in pUC:35S, ampicillin resistance | This study |
| pOsMAP5 | OsMAP5 in pUC:35S, ampicillin resistance | This study |
| pHM1 | Broad host range, resistance to spectinomycin, cos site | Hopkins et al., 1992 |
| pKMS1 | A suicide vector for marker exchange mutagenesis | Zou et al., 2011 |
| pXopZ | XopZ genomic clone in pHM1 | Song and Yang, 2010 |
| pXopN | XopN genomic clone in pHM1 | This study |
| pXopV | XopV genomic clone in pHM1 | This study |
| p35S:Xop-HA | pUC:35S plasmids individully expressing each of 17 non-TALE effector genes as indicated in text | |
| Escherichia coli | ||
| DH5α | F′ recA, ϕ80dlacZ, ΔM15 | Stratagene, CA |
| XL1-Blue MRF’ | F′ proAB, lacIqZ, ΔM15, Tn10 (Tetr) | Stratagene, CA |
| C2110 | Nalr,Rfr (polA1, rha, his) | Leong et al., 1982 |
| BL21 Star (DE3)pLysS | F- ompThsdSB (rB-mB-) gal dcm rne131 (DE3) pLysS (CamR) | Thermo Fisher Scientific, MA |
| Xanthomonas oryzae pv. oryzae | ||
| PXO99A | Philippine race 6, azacytidine resistant clone of PXO99 | Hopkins et al., 1992 |
| ΔXopZ | XopZ knockout of PXO99A | Song and Yang, 2010 |
| ΔXopN | XopN knockout of PXO99A | This study |
| ΔXopV | XopV knockout of PXO99A | This study |
| ΔXopZ;N;V | Triple knockout of XopZ, XopN and XopV in PXO99A | This study |
| ΔXopZ;N;V(XopZ) | ΔXopZ;N;V transformed with plasmid borne XopZ | This study |