Table 1.

Decellularization protocols

Group	Decellularization protocol
Group 1	LYP
Group 2	LYP+DNase (150 U/mL)+MgSO4 (50 mmol)+LYP
Group 3	LYP+deoxycholic acid+DNase+MgSO4+LYP
Group 4	LYP+deoxycholic acid+Triton X-100+DNase+MgSO4+LYP
Group 5	LYP+SDS+1% (w/v) Triton X-100+LYP
Group 6	LYP+SDS+PBS+DNase+LYP
Group 7	Control:untreated trachea

Approximately 3.5-cm-long tracheas from 42 New Zealand rabbits were separated into seven groups each containing six tracheas.

LYP, lyophilization; DNase, deoxyribonuclease; SDS, sodium dodecyl sulfate; PBS, phosphate buffered saline.