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. 2018 Nov 14;38(1):e99819. doi: 10.15252/embj.201899819

Figure 3. Freezing phenotypes of egr2‐3 mutant and EGR2‐overexpressing plants.

Figure 3

  • A–C
    Freezing phenotype (A), survival rate (B), and ion leakage (C) of the egr2‐3 mutant and egr2/EGR2:EGR2‐Myc complementation lines under non‐acclimated (NA) and acclimated (CA; 4 days at 4°C) conditions. Two‐week‐old seedlings grown on MS medium containing 0.8% agar were treated at −5°C for 0.5 h (NA) or −8°C for 0.5 h (CA).
  • D–F
    Freezing phenotype (D), survival rate (E), and ion leakage (F) of EGR2‐overexpressing plants. Two‐week‐old Super:EGR2‐Myc plants were treated as described in (A–C).
  • G–J
    Expression of CBFs (G, I) and their target genes (H, J) in egr2‐3 mutant and EGR2‐overexpressing plants. Two‐week‐old seedlings were treated at 4°C for the indicated period and subjected to qRT–PCR analysis.
Data information: In (B, C, E–J), each bar represents the mean ± SEM of three independent experiments, each of which has three technical repeats. Asterisks indicate significant differences compared to the wild type with the same treatment (*P < 0.05, **P < 0.01, two‐tailed t‐test).