Figure 4. Increased IL‐17 and IL‐22 production in T‐bet^−/− lungs infected with influenza virus or superinfected with bacteria.

• A
  Concentrations of cytokines or chemokines in the BAL of days 5 and 9 post‐sub‐lethal influenza virus infection as determined by protein bead array. See also Fig EV4A and B.
• B, C
  Mice were superinfected as described in Fig 3A. (B) Concentrations of IL‐17 and IL‐22 in BAL of mice, as determined by ELISA. See also Fig EV3E. (C) Concentrations of IL‐17 and IL‐22 in BAL of mice 12 h after infection with 2 × 10⁵ cfu of Streptococcus pneumoniae serotype 19F alone (−Flu), or following superinfection (+Flu), assessed by ELISA.

Data information: Data in (A) are obtained from one independent experiment with four mice per group, and results of selected analytes IFNγ, CXCL10, IL‐6, IL‐13 and IL‐17 are reconfirmed in a separate independent experiment by a customized protein bead array. Data in (B) and (C) are representative of two independent experiments with 3–4 mice per group. All data are represented as mean ± SEM. Significance in (A–C) was determined by unpaired Student's t‐test. n.s., not significant.