Table 1.
Porcine reproductive and respiratory syndrome virus (PRRSV)-1 detection in air samples collected in experimental chamber.
| Air Sampler | Time Point | qRT-PCR Copies/mL (Ct-Values) | Virus Isolation |
|---|---|---|---|
| t-valueCoriolis®μ | During nebulisation | 1.8 × 108 (20.74) | 1.5 × 10 TCID50 |
| 1 h after nebulisation | 4.7 × 107 (22.82) | negative | |
| MD8 Airscan | During nebulisation | 1.5 × 107 (24.54) | negative |
| 1 h after nebulisation * | 5.5 × 106 (26.07) | negative | |
| IOM Multidust sampler 1 | 2 h after start of nebulisation | 6.3 × 105 (29.37) | negative |
| IOM Multidust sampler 2 | 2 h after start of nebulisation | 5.2 × 105 (29.64) | negative |
Results of qRT-PCR and virus isolation performed on air samples collected by the three air sampling systems under experimental conditions in an experimental chamber. * Sampling time was seven minutes due to a switch-off of the sampler. TCID: tissue culture infective dose. Ct-value: threshold cycle value.