Figure 3.

Two weeks of Bronze-enriched diet induced Slpi and Hmox1 expression in the medial colon of WT and Winnie mice. Histograms represent the average expression of Il-10, Tnf-α, Ifnγ, Slpi and Hmox1 (A–E respectively) measured by real time PCR in the medial colon of WT and Winnie mice after two weeks of Control- (striped bars) and Bronze-enriched diet (white bars). Black bars represent the gene expression at Day 0. All bars represent mean expression ± SEM for each treatment. Control and Bronze dependent expression were compared to WT Day 0 for the statistical evaluation. Student’s t-test was used to compare every measurement to the corresponding WT Day 0 and evaluate the significance of the data. ** P < 0.01,* P < 0.05 (Student’s t-test). Grouped analyses were performed with the two-way ANOVA test, using Bonferroni as a post test.