Table 3.
Sequence of the primers used for molecular identification of Aspergillus section Flavi isolates.
Gene | Gene Name | Length (bp) | Primers | Sequence (Nucleotides: 5’→3’) |
|
---|---|---|---|---|---|
Forward | Reverse | ||||
ITS (4–5) | Internal transcribed spacer | 300–330 | ITS5 | 5′-GGAAGTAAAAGTCGTAACAAGG | |
ITS 4 | 5′-TCCTCCGCTTATTGATATGC | ||||
benA-2 | ß-tubulin | 1125 | benA 2a | 5′-GGTAACCAAATCGGTGCTGC | |
benA 2b | 5′-ACCCTCAGTGTAGTGACCCTTGGC | ||||
cmdA | Calmodulin | 543 | Cmd5 | 5′-CCGAGTACAAGGAGGCCTTC-3’ | |
Cmd6 | 5′-CCGATAGAGGTCATAACGTGG-3’ |