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. 2018 Nov 26;16(12):466. doi: 10.3390/md16120466

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The flow cytometry results of annexin V-FITC/PI double staining of HL-60 cells after 4 h of incubation with PM III (at IC₅₀ and 2xIC₅₀) in the presence or absence of the caspase inhibitor, Z-VAD-FMK. Columns, from left to right: Control (untreated cells); 2.5 μM PM III; 5 μM PM III; 50 μM apoptotic inducer, campthotecin. Top row: No Z-VAD-FMK added, bottom row: 50 μM Z-VAD-FMK added. The results are presented as the percentage of viable (AV−PI−), apoptotic (AV+PI−), and late apoptotic/necrotic (AV+PI+) cells.