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. 2018 Dec 17;16(12):512. doi: 10.3390/md16120512

Figure 1.

Figure 1

In vitro fat-lowering effect, adipose tissue gene expressions, and anti-hypertensive activity of wakame (W), carob pod (CP), and its combination (W+CP). (A) Mature 3T3-L1 adipocytes were treated with 95% ethanol (control) or W (1 or 10 µg/mL), CP (50 or 100 µg/mL), and W+CP (1 µg/mL + CP 50 µg/mL or 10 µg/mL + CP 50 µg/mL) extracts diluted in 95% ethanol for 24 h, and TG content was analyzed. Ethanol concentration in the medium never exceeded 0.1%. Values of three independent experiments carried out in sextuplicate are expressed as mean ± SEM of the reduction percentage compared with the control group (Y-axis). Data not sharing a common letter are significantly different (p < 0.01). (B) mRNA levels of genes related to de novo lipogenesis, glucose uptake, apoptosis, and TG assembly were measured. Data are presented as fold change mean ± SEM of three independent experiments carried out in sextuplicate, t < 0.1, ** p < 0.01, n.d.: not detected. (C) Angiotensin-converting enzyme (ACE) inhibitory activity was evaluated. Values of three independent experiments carried out in triplicate are expressed as a percentage mean of activity change ± SEM. Data not sharing a common letter are significantly different (p < 0.01).