Figure 2.

In vitro effect of wakame (W), carob pod (CP), and its combination (W+CP) on the activity of enzymes involved in de novo lipogenesis and glycerol release. (A) Mature 3T3-L1 adipocytes were incubated with 95% ethanol (control) or W 1 µg/mL + CP 50 µg/mL extracts diluted in 95% ethanol for 24 h, and Western blotting was used to determine the cleaved FAS, phosphorylated ACC (serine 79), and total ACC protein expression. (B) β-actin was used as a loading control, and the quantified expression levels of three independent experiments carried out in duplicates (percentage mean ± SD) by Image software were plotted in the bar graphs. FAS activity (C) and glycerol release (D) to the medium were determined using spectrophotometry. Values of the three independent experiments carried out in duplicate are expressed as mean ± SEM.