Table 2.

Primers for PCR amplification of each gene studied.

Primers	Sense Primer	Antisense Primer	Ref.	Annealing T(°C)
β-actin	5′-ACG AGG CCC AGA GCA AGA G-3′	5′-GGT GTG GTG CCA GAT CTT CTC-3′		60.0
bax	5′-GTG AGC GGC TGC TTG TCT-3′	5′-GTG GGG GTC CCG AAG TAG-3′		59.0
bcl2	5′-AGT ACC TGA ACC GGC ATC TG-3′	5′-GGG GCC ATA TAG TTC CAC AAA-3′		59.0
cox2	5′-GCT ACC TGG AGT ACA TGA AG-3′	5′-CTG TGA CTC CAG CTT ATC TG-3′	[43]	57.3
il6	5′-GCT ACC TGG AGT ACA TGA AG-3′	5′-GCA TAA CGC ACT AGG TTT GCC-3′	[44]	60.0
nos2	5′-CTT TGC CAC GGA CGA GAC-3′	5′-TCA TTG TAC TCT GAG GGC TGA C-3′		63.7
scd1	5′-TTC TTG CGA TAC ACT CTG GTG C-3′	5′-CGG GAT TGA ATG TTC TTG TCG T-3′	[45]	64.5
tnfa	5′-TCA CCT GCT GCT ACT CAT TC-3′	5′-TAC AGA AGT GCT TGA GGT GG-3′	[43]	62.7

bax, BCL2-associated X protein; bcl2, B cell leukemia/lymphoma 2; cox2, cyclooxygenase 2; il6, interleukin 6; nos2; nitric oxide synthase 2; scd1, stearoyl-Coenzyme A desaturase 1; tnfα, tumor necrosis factor α.