. 2018 Oct 22;6(4):98. doi: 10.3390/biomedicines6040098

Table 1.

Yields and purities of [azasulfuryl⁴]-GHRP 6 analogs.

Azasulfurylpeptides	Crude Purity (%) ^a	Isolated Purity (%) ^b	Isolated Yields (%) ^c	HRMS
Azasulfurylpeptides	Crude Purity (%) ^a	Isolated Purity (%) ^b	Isolated Yields (%) ^c	m/z (cal)	m/z (obs)
His-D-Trp-Ala-AsF-D-Phe-Lys-NH₂ (3a)	73%	>99%	12%	893.3851	893.3832
His-D-Trp-Ala-AsY-D-Phe-Lys-NH₂ (3b)	70%	>99%	9%	909.3801	909.3805
His-D-Trp-Ala-(4-F)AsF-D-Phe-Lys-NH₂ (3c)	65%	>99%	3%	911.3757	911.3769
His-D-Trp-Ala-(4-MeO)AsF-D-Phe-Lys-NH₂ (3d)	75%	>99%	10%	923.3957	923.3936
His-D-Trp-Ala-2-AsNal-D-Phe-Lys-NH₂ (3e)	61%	>99%	10%	943.4008	943.4007
Ala-D-Trp-Ala-(4-F)AsF-D-Phe-Lys-NH₂ (3f)	79%	>99%	23%	845.3647	845.3539

^a Crude purities of peptide obtained after an aliquot cleavage of resin using TFA:H₂O:TES (95:2.5:2.5), followed by LC-MS analysis using 5–80% MeOH (0.1% FA) in H₂O (0.1% FA) as eluant. ^b Isolated purities of peptide obtained by a LC-MS analysis using two separate gradients of MeOH (0.1% FA) in H₂O (0.1% FA) or MeCN (0.1% FA) in H₂O (0.1% FA). ^c Isolated yields calculated from resin loading.