Figure 2.

Inhibition, by VLCDCA 28:4, of lipopolysaccharide (LPS) (1 µg/mL of media) stimulation of nitric oxide (NO) release into the medium of THP-1 monocytes incubated for 48 h. in 24 well plates. NO was measured as nitrite in the medium using the Griess colorimetric procedure⁴⁰. The 0 concentration point represents the NO production by LPS, while other points represent LPS and coincubation with VLCDCA 28:4 in ethanol. The control and LPS wells also received an equal volume of ethanol. Values are the mean values of 8 wells, with the RSD ranging from 3.8 to 10.2%.