Figure 9.

Effects of low EGF dose on endocytosis in cells with CBL alterations. (A) HeLa cells were transfected with dominant-negative CBL (70z-YFP) and treated with nocodazole (200 ng/mL) for 16 h. Cells were then treated with low-dose EGF (2 ng/mL) for the indicated times. Cells were stained with EGFR pY1086 (red) and DAPI (blue). * represents interphase cells, # represents mitotic cells, and ’ represents transfected cells. (B) Quantification of plasma membrane retainment of YFP for (A) for at least 10 cells (see Materials and Methods). The error bars are the SEMs. (C) Cells transfected with CBL-YFP were used as control.