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A proposed schematic of a method to assess human daily water requirements by measuring the intensity of neuroendocrine responses that are employed by the brain to defend homeostasis of body water volume and concentration. These responses and thresholds are inherent hydration biomarkers, and the means by which the brain maintains good health and optimal function. Abbreviations: AVP, arginine vasopressin; ANG II, angiotensin II; ALD, aldosterone; ANP, atrial naturietic peptide.
