Figure 2.

Maximum likelihood tree reconstructed using near full-length genomes of classical insect-specific flaviviruses (cISFs). The Brazilian strains described in this study are indicated by arrows. Values in the tree indicate the statistical support of each node, each of which was calculated using the approximate likelihood ratio test (aLRT). The topology shows that three strains (Macapá 01, 02 and 04) are closely related to cell fusing agent virus (CFAV) and the other two (Macapá 05 and 06) to Culex flavivirus (CxFV). The scale bar under the tree represents the nucleotide substitutions per site.