Table 3.

Concentration of ^natZn, ⁶⁸Zn and total Zn (^natZn + ⁶⁸Zn) (in μg Zn·g⁻¹ protein) found in Zn binding-proteins/molecules, i.e., MTs, other than MTs and all Zn-binding proteins/molecules, in the water-soluble protein fraction of HRPEsv cells by ID-, IPD-SEC-ICP-MS. HRPEsv cells were either not treated (control) or exposed to ⁶⁸ZnSO₄ at 25, 50 or 100 μM, separately, for 24 h following treatment with 5 mM of AAPH for 1 h.

Treatment	Zn-Binding Proteins	natZn	68Zn	Total Zn (natZn + 68Zn)
		(μg·g−1 Protein)	(μg·g−1 Protein)	(μg·g−1 Protein)
Control	Zn-MTs	12.0 ± 2.8	0.00 ± 0.00	12.0 ± 2.8
	Other Zn-binding proteins	28.3 ± 9.5	0.00 ± 0.00	28.3 ± 9.5
	All Zn binding proteins/molecules	40.3 ± 12.3	0.00 ± 0.00	40.3 ± 12.3
AAPH	Zn-MTs	11.2 ± 2.4	0.00 ± 0.00	11.2 ± 2.4
	Other Zn-binding proteins	13.5 ± 0.1	0.00 ± 0.00	13.5 ± 0.1
	All Zn binding proteins/molecules	24.7 ± 2.5	0.00 ± 0.00	24.7 ± 2.5
25 µM 68Zn + AAPH	Zn-MTs	6.9 ± 0.8	34.5 ± 5.6	41.4 ± 6.4
	Other Zn-binding proteins	9.0 ± 3.2	18.2 ± 11.6	27.2 ± 14.8
	All Zn binding proteins/molecules	15.9 ± 4.0	52.6 ± 17.2	68.5 ± 21.2
50 µM 68Zn + AAPH	Zn-MTs	17.5 ± 0.5	131.9 ± 4.0	149.4 ± 4.5
	Other Zn-binding proteins	7.9 ± 0.3	17.6 ± 0.8	25.5 ± 1.2
	All Zn binding proteins/molecules	25.4 ± 0.9	149.5 ± 4.8	174.9 ± 5.7
100 µM 68Zn + AAPH	Zn-MTs	16.2 ± 0.4	532.3 ± 16.3	548.5 ± 15.9
	Other Zn-binding proteins	6.9 ± 0.9	50.4 ± 33.2	57.4 ± 34.2
	All Zn binding proteins/molecules	23.1 ± 1.3	582.7 ± 49.5	605.9 ± 50.8