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. 2018 Dec 2;10(12):508. doi: 10.3390/toxins10120508

Figure 3.

Figure 3

FabC11:Stx2 protects HK-2 from Stx2-induced morphological disturbances. HK-2 seeded on glass coverslips was pre-treated with FabC11:Stx2 (10 µg/mL, 1 h at 37 °C), and Stx2 (0.1 ng/mL) was then added. Co-incubation was performed by adding FabC11:Stx2 (10 µg/mL) with Stx2 (0.1 ng/mL) simultaneously and incubating cells for 72 h. Finally, cells were stained with H&E, and the morphology (A) and number of HK-2 (B) were analyzed by light microscopy (×200 and ×400). HK-2 areas (C) were measured using ImageJ software. The black arrows indicate the morphological changes, namely intracellular edema (thick arrows) and elongated shape (thin arrows). Results are expressed as means ± SEM of three experiments. One hundred percent represents values of controls. Stx2 vs. Ctrl, * p < 0.05. Pre/co-incubation vs. Stx2, # p < 0.05.