Figure 5.

Denaturation of myoglobin monitored by CD spectroscopy—Denaturation of myoglobin (0.15 mg/mL) by EBB was monitored by change in CD signal around the heme transition. Experiments were performed in the presence of 0 (black), 9.4 mM (green), 28.1 mM (blue), or 56.3 mM (red). (A) NaCl; (B) LiBF₄; (C) BMICl; (D) EMIAc; or (E) BMIBF₄. Normalization was performed by setting the CD signal at 409 nm to 1 at 0 detergent concentration. Panel (F) shows representative CD spectra for myoglobin with (green) and without EBB (black) in buffer or with (red) or without (blue) EBB in the presence of 56.3 mM BMIBF₄.