Figure 1.

H1993-induced bone lesions were inhibited in Tgfbr2^LysMCre knockout (KO) mice. (A) Quantification of bone lesion areas in the control and KO mouse tibiae. (B) Representative bone lesions (red outlines) shown by weekly X-ray images. (C) Hematoxylin and eosin (H&E) staining images (4×) confirm tumor growth in the mouse tibiae (red outlines) at 4 weeks (wk) post-injection. (D) Representative mouse tibiae at 4 wk post-injection, stained for CD31 (20×), Ki67 (20×), or TRAP (10×). Mean ± SD, n ≥ 10 per group. *** p < 0.001, ** p < 0.01, * p ≤ 0.05, by linear mixed-effect models for bone lesions and Student’s t-test for paired comparison.