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. 2018 Dec 17;8(12):1065. doi: 10.3390/nano8121065

Figure 1.

Figure 1

Characterization of the polydopamine particles, containing transferrin (PDA/Tf), polydopamine/human serum albumin (PDA/HSA), and PDA/Tf-A488 nanoparticles (NPs). (A) Photograph of an aqueous suspension of PDA/HSA NPs, and (B) absorbance spectra of the PDA/HSA with the subtracted background of bare PDA NPs (black line), and absorbance spectra of the HSA alone (green line). (C) The absorbance spectra of the PDA/Tf with subtracted background of the bare PDA NPs (black line), absorbance spectra of the Tf alone (green line), and (D) the absorbance spectra of the PDA/Tf-A488 (black line). The dotted lines represent typical absorbance maxima for the proteins (270–280 nm) and the Alexa 488 fluorophore (495 nm), in panels (B,C). (E) The absorbance measurement of the bare PDA NPs, PDA/HSA NPs, and PDA/Tf NPs, at 280 nm. The difference in absorbance of the bare PDA NPs and the PDA/protein NPs can be attributed to the proteins present. (F) Scanning electron micrograph of the drop-casted PDA/Tf NPs, and (G) scanning electron micrograph of the drop-casted PDA/HSA NPs. Scale bar = 500 nm.