Figure 5.

Rate of apoptosis induction in the B16F10 mouse melanoma cells exposed to the PDA/protein NPs, upon light irradiation. The melanoma cells were exposed to 20 μg/mL PDA/Tf NPs (A–C and Supplementary Video 4), PDA/HSA NPs (D–F and Supplementary Video 5), or media only (G–I and Supplementary Video 6). Similar to Figure 3, two regions, per field, were defined, one irradiated by 405 nm light (purple square) and one with 633 nm light (red square). The addition of the Annexin V dye allowed for the analysis of the apoptosis onset. Different time points are presented: t = 0 (A,D,G), t = 1 h (B,E,H), and t = 2 h (C,F,I). All datasets have an n value of at least three.