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. 2018 Dec 17;8(12):1065. doi: 10.3390/nano8121065

Figure 8.

Figure 8

Light-induced apoptosis using melanoma spheroid models. The development of the spheroid model is depicted in the representative images in panel (A), corresponding to the increasing time-points during their growth. Representative phase contrast image of the spheroid model with the selected regions of elevated irradiation with 405 nm (violet) and 633 nm (red) laser light (B). Rendered cLSM z-stack of the stained B16F10 assembled into the spheroid model, after 24 h of development (C). Analyzed frames of the videos showing the B16F10 spheroids, exposed to the PDA/Tf NPs, at time point 0 (D), after 2.5 h (E), and 4.5 h (F), and spheroids exposed to the PDA/HSA NPs, at time point 0 (G), after 2.5 h (H), and 4.5 h (I). Every frame of the videos was analyzed and the Annexin V intensity per irradiated area, either 405 (violet) or 633 (red), was recorded and is shown in panel (J). The blue line represents the cell-death rate of the samples treated with the PDA/Tf NPs, the violet line is that of the samples treated with the PDA/HSA NPs, and both were irradiated with a 405 nm laser light. The red line represents the cell-death rate of the samples treated with the PDA/Tf NPs, the green line shows the samples treated with the PDA/HSA NPs (both irradiated with 633 nm laser light). N of PDA/Tf = 2 and N of PDA/HSA = 3.