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Determination of the C-terminal residue of the MoCV1-A 60 kDa protein (labeled P58 in the figure). The C-terminus of P58 is a truncated version of the ORF3 protein missing 200 amino acids. Mass spectrometry of tryptic peptides derived from P58 identified peptides specific to the deduced amino acid sequence of ORF3, but no peptide sequence mapped to the C terminus of the deduced amino acid sequence of ORF3 (M565 to L799).
