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Release of mycoviruses from the mycelium of mycovirus-infected M. oryzae into the culture supernatant. The mycoviruses gradually appeared and accumulated in the liquid medium during the long-term suspension culture. After five weeks of liquid culture, 250 μL samples of the culture supernatant were subjected to stepwise centrifugation treatments. Total nucleic acids were extracted from each supernatant and then subjected to agarose gel electrophoresis.
