Table 2.
The relative abundance of long chain fatty acids in the faecal extracts based on analysis of a ZICp HILIC column.
| Mass | RT | Putative Metabolite | p-Value HCPA | PA/HC | p-Value HCPB | PB/HC | p-Value HCPC | PC/HC | p-Value HCPD | D/HC | p-Value HCPE | E/HC |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 254.2246 | 3.6 | Hexadecenoic acid | 0.005 | 2.334 | 0.004 | 3.028 | 0.001 | 3.219 | 0.027 | 2.287 | 0.035 | 1.623 |
| 256.2401 | 3.6 | Hexadecanoic acid | 0.839 | 1.038 | 0.860 | 1.038 | 0.957 | 1.011 | 0.522 | 0.866 | 0.249 | 1.212 |
| 258.1829 | 3.6 | Tetradecanedioic acid | 0.016 | 0.362 | 0.010 | 0.332 | 0.007 | 0.300 | 0.068 | 0.507 | 0.637 | 1.200 |
| 258.2198 | 3.5 | Hydroxypentadecanoic acid | 0.984 | 1.005 | 0.713 | 1.143 | 0.401 | 1.379 | 0.266 | 1.448 | 0.716 | 1.095 |
| 260.1988 | 3.3 | Dihydroxytetradecanoic acid | 0.891 | 0.935 | 0.051 | 0.240 | 0.037 | 0.180 | 0.034 | 0.161 | 0.241 | 1.512 |
| 266.1882 | 3.4 | Hydroxyhexadecatrienoic acid | 0.537 | 0.839 | 0.007 | 0.391 | 0.015 | 0.459 | 0.029 | 0.506 | 0.192 | 1.387 |
| 268.2036 | 3.1 | Hydroxyhexadecadienoic acid | 0.016 | 0.376 | 0.001 | 0.118 | 0.001 | 0.113 | 0.002 | 0.176 | 0.552 | 0.852 |
| 270.2195 | 3.5 | Hydroxyhexadecenoic acid | 0.940 | 1.024 | 0.174 | 0.702 | 0.846 | 1.081 | 0.902 | 0.963 | 0.095 | 1.472 |
| 272.2351 | 3.5 | Hydroxyhexadecanoic acid | 0.905 | 0.947 | 0.228 | 0.578 | 0.389 | 0.690 | 0.538 | 0.785 | 0.449 | 1.287 |
| 278.2245 | 3.5 | Octadecatrienoic acid | 0.004 | 0.115 | 0.005 | 0.143 | 0.004 | 0.135 | 0.004 | 0.128 | 0.014 | 0.293 |
| 280.2401 | 3.6 | Octadecadienoic acid | 0.212 | 0.513 | 0.220 | 0.517 | 0.211 | 0.508 | 0.098 | 0.342 | 0.195 | 0.500 |
| 282.2559 | 3.6 | Octadecenoic acid | 0.826 | 1.093 | 0.374 | 0.666 | 0.839 | 1.102 | 0.407 | 0.688 | 0.065 | 1.871 |
| 284.2713 | 3.5 | Octadecanoic acid | 0.399 | 0.756 | 0.022 | 0.400 | 0.020 | 0.394 | 0.018 | 0.374 | 0.928 | 0.976 |
| 288.23 | 3.2 | Dihydroxyhexadecanoic acid | 0.810 | 0.893 | 0.047 | 0.252 | 0.048 | 0.256 | 0.052 | 0.269 | 0.296 | 1.436 |
| 296.2349 | 3.5 | Hydroxyoctadecadienenoic acid | 0.004 | 0.285 | 0.004 | 0.271 | 0.004 | 0.269 | 0.004 | 0.279 | 0.235 | 0.700 |
| 298.2506 | 3.6 | Hydroxyoctadecenoic acid | 0.797 | 1.105 | 0.509 | 0.784 | 0.921 | 1.038 | 0.950 | 1.023 | 0.019 | 2.064 |
| 304.2401 | 3.5 | Eicosatetraenoic acid | 0.088 | 18.052 | 0.100 | 4.904 | 0.049 | 2.915 | 0.228 | 1.968 | 0.008 | 3.448 |
| 306.2558 | 3.5 | Eicosatrienoic acid | 0.002 | 16.182 | 0.008 | 13.854 | 0.014 | 9.671 | 0.049 | 8.821 | 0.003 | 8.751 |
| 308.2715 | 3.5 | Eicosadienoic acid | 0.028 | 8.716 | 0.041 | 6.338 | 0.017 | 6.119 | 0.008 | 5.658 | 0.000 | 3.709 |
| 310.2145 | 3.5 | Dihydroxyoctadecatrienoic acid | 0.042 | 0.593 | 0.691 | 0.890 | 0.368 | 0.783 | 0.017 | 0.496 | 0.676 | 1.147 |
| 310.2871 | 3.5 | Eicosenoic acid | 0.045 | 1.793 | 0.308 | 1.315 | 0.422 | 1.181 | 0.772 | 1.076 | 0.020 | 1.588 |
| 312.2301 | 3.6 | Dihydroxyoctadecadienoic acid | 0.871 | 1.053 | 0.868 | 1.053 | 0.860 | 0.947 | 0.191 | 0.647 | 0.350 | 1.244 |
| 312.2663 | 3.5 | Hydroxynonadecenoic acid | 0.232 | 1.695 | 0.013 | 2.360 | 0.018 | 2.560 | 0.045 | 2.148 | 0.733 | 1.149 |
| 312.3028 | 3.5 | Eicosanoic acid | 0.851 | 1.071 | 0.203 | 1.595 | 0.082 | 2.166 | 0.304 | 1.614 | 0.766 | 1.085 |
| 330.2405 | 3.7 | Trihydroxyoctadecenoic acid | 0.175 | 0.508 | 0.373 | 1.590 | 0.646 | 1.220 | 0.878 | 0.938 | 0.583 | 1.261 |
| 332.2716 | 3.5 | Docosatetraenoic acid | 0.006 | 20.326 | 0.004 | 9.694 | 0.003 | 6.946 | 0.006 | 6.532 | <0.001 | 8.116 |
| 334.2144 | 3.7 | Dihydroxyeicosapentaenoic acid | 0.964 | 1.024 | 0.938 | 1.043 | 0.661 | 0.775 | 0.605 | 0.743 | 0.233 | 0.414 |
| 334.2871 | 3.5 | Docosatrienoic acid | 0.127 | 1.701 | 0.491 | 1.457 | 0.856 | 1.098 | 0.405 | 1.491 | 0.201 | 1.664 |
| 336.3029 | 3.5 | Docosadienoic acid | 0.738 | 1.226 | 0.444 | 0.705 | 0.037 | 0.341 | 0.116 | 0.488 | 0.880 | 0.939 |
| 338.3186 | 3.5 | Docosenoic acid | 0.119 | 1.665 | 0.768 | 0.935 | 0.192 | 0.759 | 0.185 | 0.728 | 0.039 | 1.459 |
| 340.334 | 3.4 | Docosanoic acid | 0.610 | 1.264 | 0.017 | 0.320 | 0.019 | 0.342 | 0.026 | 0.356 | 0.262 | 1.570 |
| 342.2769 | 3.4 | Eicosanedioic acid | 0.021 | 0.311 | 0.061 | 0.454 | 0.069 | 0.468 | 0.088 | 0.504 | 0.331 | 0.718 |
| 346.2353 | 3.9 | Tetrahydroxyoctadecenoic acid | 0.046 | 0.447 | 0.301 | 0.691 | 0.168 | 0.618 | 0.244 | 0.670 | 0.978 | 0.992 |
| 352.3341 | 3.4 | Tricosenoic acid | 0.164 | 1.402 | 0.006 | 2.492 | 0.005 | 2.596 | 0.127 | 1.788 | 0.088 | 1.392 |
| 354.2408 | 3.7 | Trihydroxyeicosatetraenoic acid | 0.799 | 0.938 | 0.254 | 0.697 | 0.078 | 0.598 | 0.239 | 0.730 | 0.097 | 1.433 |
| 354.3134 | 3.4 | Hydroxydocosenoic acid | 0.209 | 0.563 | 0.022 | 0.361 | 0.036 | 0.419 | 0.088 | 0.529 | 0.602 | 1.160 |
| 354.3498 | 3.4 | Tricosanoic acid | 0.999 | 1.000 | 0.011 | 0.351 | 0.019 | 0.427 | 0.019 | 0.408 | 0.511 | 1.225 |
| 356.329 | 3.4 | Hydroxydocosanoic acid | 0.494 | 0.713 | 0.011 | 0.239 | 0.013 | 0.270 | 0.021 | 0.325 | 0.461 | 0.763 |
| 364.3342 | 3.4 | Tetracosadienoic acid | 0.037 | 4.794 | 0.214 | 2.076 | 0.651 | 1.220 | 0.312 | 2.154 | 0.048 | 3.661 |
| 370.2358 | 3.8 | Tetrahydroxyeicosatrienoic acid | 0.456 | 0.840 | 0.044 | 0.532 | 0.012 | 0.464 | 0.039 | 0.536 | 0.075 | 1.544 |
| 372.2509 | 3.8 | Tetrahydroxyeicosadienenoic acid | 0.086 | 0.574 | 0.030 | 0.537 | 0.004 | 0.402 | 0.016 | 0.497 | 0.080 | 1.459 |
| 382.2719 | 3.6 | Dihydroxydocosatrienoic acid | 0.039 | 0.268 | 0.101 | 0.379 | 0.041 | 0.278 | 0.140 | 0.459 | 0.131 | 0.485 |
| 382.3447 | 3.3 | Hydroxy tetracosanoic acid | 0.556 | 0.702 | 0.090 | 0.282 | 0.084 | 0.270 | 0.121 | 0.349 | 0.835 | 1.103 |
(HC) Healthy control children, (PA) CD children pre-EEN treatment, (PB) CD children 15 days post-EEN treatment, (PC) CD children 30 days post-EEN treatment, (PD) CD children 60 days post-EEN treatment, (PE) CD children back to a free diet.