Table 2.
Plasmids used in this study.
| Plasmids | Relevant Characteristics | Reference |
|---|---|---|
| pET-Vip | Vip3Aa gene cloned into pET-28a (+), His tag binding C-terminal of Vip3Aa | [9] |
| pET-Vip-flag | Vip3Aa gene cloned into pET-28a (+), Flag-His tag binding C-terminal of Vip3Aa | [9] |
| pET-ChiB-flag | ChiB gene cloned into pET-28a (+), Flag-His tag binding C-terminal of ChiB | [9] |
| pET-Vip-RFP | RFP gene cloned into pET-Vip, RFP binding C-terminal of Vip3Aa | [9] |
| pIZT/V5-His | Expression vector, Zeocinr, C-terminal V5-His tag | Invitrogen |
| pIZT-Fgfr | Sf-Fgfr gene cloned into pIZT/V5-His, V5-His tag binding C-terminal of Sf-Fgfr | This study |
| pIZT-fgi1 | Fragment of Sf-Fgfr gene (60-679) and the reverse complemented fragment of Sf-Fgfr (559-60) cloned into pIZT/V5-His | This study |
| pIZT-fgi2 | Fragment of Sf-Fgfr gene(1600-2219) and the reverse complemented Fragment of Sf-Fgfr (2099-1600) cloned into pIZT/V5-His | This study |
| pGEX-6P-1 | Expression vector, Ampr, N-terminal GST tag | Lab collection |
| pGEX-Fgfr-N | Extracellular sequence of Sf-Fgfr gene (Fgfr-N) cloned into pGEX-6P-1, GST tag binding N-terminal of Fgfr-N | This study |
Ampr: ampicillin resistance, Kanr: kanamycin resistance, Zeocinr: zeocin resistance.