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Comparison of (a) normal and (b) Adriamycin (ADR)-treated MCF7 cells stained for SA-β-gal activity. ADR-treated cells were larger and had a flatter morphology than untreated cells. Cell counting (250 cells per condition) showed that the number of SA-β-gal-positive (blue) cells versus the number of total cells was approximately 4% in the untreated cells and ~60% in the treated cells.
