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. 2018 Dec 11;10(12):507. doi: 10.3390/cancers10120507

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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ITG β4 degradation by ARRDC3 is associated with lysosomal targeting of endosomal ITG β4. (A) MDA-MB-231 cells were transfected with HA-ARRDC3 plasmid. Next day, the cells were incubated with LysoTracker^® Red DND-99 (red) before EGF treatment (20 ng/μL) for the indicated time periods. The cells were fixed and stained with fluorescence-labeled antibodies against HA (ARRDC3; blue) and ITG β4 (green). Scale bar: 50 μm. (B) MCF-7 cells were incubated with LysoTracker^® Red DND-99 (red) before EGF treatment (100 ng/μL) for the indicated time periods. The cells were fixed and stained with fluorescence-labeled antibodies against ITG β4 (green). All images were captured by a fluorescence microscope with DIC optic. Scale bar: 50 μm. Representative images were selected from three independent experiments.

ITG β4 degradation by ARRDC3 is associated with lysosomal targeting of endosomal ITG β4. (A) MDA-MB-231 cells were transfected with HA-ARRDC3 plasmid. Next day, the cells were incubated with LysoTracker^® Red DND-99 (red) before EGF treatment (20 ng/μL) for the indicated time periods. The cells were fixed and stained with fluorescence-labeled antibodies against HA (ARRDC3; blue) and ITG β4 (green). Scale bar: 50 μm. (B) MCF-7 cells were incubated with LysoTracker^® Red DND-99 (red) before EGF treatment (100 ng/μL) for the indicated time periods. The cells were fixed and stained with fluorescence-labeled antibodies against ITG β4 (green). All images were captured by a fluorescence microscope with DIC optic. Scale bar: 50 μm. Representative images were selected from three independent experiments.