Figure 2.

DUSP6 prevented the Aβ-triggered decrease in NSC viability. (A) DUSP6 mRNA was overexpressed after it was transfected with DUSP6-overexpressed plasmid pcDNA-DUSP6. pcDNA3.0 empty vector transfection showed no effect on DUSP6 expression in NSCs; (B) NSCs were either pre-transfected with DUSP6 overexpression plasmid or not transfected. Both groups were treated with 5 μM Aβ25–35 for 24 h. Cell viability assessed by CCK8 assay showed no significant change in Aβ-treated NSC vitality after DUSP6 transfection. N.S.: p > 0.05, **: p < 0.01, ***: p < 0.001 vs. control. *: p < 0.05 vs. Aβ-treated group. n = 3. N.S.: Not significant.