Figure 3.

Viral RNA copy numbers in the saliva of single and dual infected Ae. aegypti mosquitoes. CHIKV copy numbers in mosquito saliva are shown for (a) 7 dpi and (b) 12 dpi. ZIKV copy numbers in mosquito saliva are shown for (c) 7 dpi and (d) 12 dpi. All viral copy numbers were quantified using qRT-PCR and are shown combined from three replicate experiments. The median, including zero values log10(x + 1) transformed, is indicated, and error bars show the interquartile range. ANOVA was used to determine statistical significance as indicated (* p < 0.05; ** p < 0.01; and *** p < 0.001), taking replicates into account.